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Use of comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometric detection for metabolomic analysis of Gloeophyllum trabeum fungus
Kuchler, Ondřej ; Sobotníková, Jana (advisor) ; Kozlík, Petr (referee)
Fungus Gloeophyllum trabeum (Agaricomytes: Gloeophyllates) is a brown rot wood-decay fungus which produces a vast spectrum of volatile secondary metabolites. Scientific publications state, that one of the metabolites produced by G. trabeum, can be the substance (3Z,6Z,8E)-dodecatrien-1-ol. This chemical substance is also the main component of trail-following pheromone of Rhinotermitidae termite family. In this diploma thesis, I was trying to verify whether various species of G. trabeum are in fact capable of producing the substance (3Z,6Z,8E)-dodecatrien-1-ol. I was also focusing on the effects of saccharides, present in nutrient solutions, on quantitative and qualitative change in composition of volatile secondary metabolites produced by G. trabeum. The saccharides I used for my research were - maltose, fructose, sucrose, xylose, and mannose. The analysis was made by using comprehensive two-dimensional gas chromatography separation technique with time-of-flight mass spectrometric detection (GC×GC-TOFMS). During my research I discovered that one of obtained species of G. trabeum can produce substance (3Z,6Z,8E)-dodecatrien-1-ol, but only under specific conditions. It is produced when cultivating on Petri dishes on agar - cellulose growth media. The measurement was further validated by...
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Analysis of a human body odour using comprehensive two-dimensional gas chromatography
Bušovská, Radka ; Sobotníková, Jana (advisor) ; Žáček, Petr (referee)
Body odour perception plays an important role in human mate choice, especially in women. It was previously proposed that women select partners whose body odour resembles that of woman's fathers. Yet, this phenomenon has only been confirmed using ethological studies based on subjective perception of body odour similarities. Therefore, the aim of my diploma thesis was to test this hypothesis instrumentally using comprehensive GC×GC-TOFMS and subsequent multidimensional analyses of body odour chemical profiles of male partners and fathers of adult women. Body odour sampling from left and right axilla of fathers and partners of 41 women (altogether 164 samples) was performed using cotton swabs, which were then frozen and extracted into hexane. Typical human volatile substances, such as hydrocarbons, carboxylic acids, esters, alcohols, aldehydes, ketones, sterols and terpenes were detected in all examined samples. Using a newly available "tile-based" chromatographic alignment algorithm, we obtained a set of 341 compounds systematically occurring in male axillary odour. The principal component analysis was used to calculate Euclidean distances for all pairs of the studied male subjects. These estimates of "chemical distances" revealed to be significantly smaller for father-partner pairs of individual...
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Use of comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometric detection for metabolomic analysis of Gloeophyllum trabeum fungus
Kuchler, Ondřej ; Sobotníková, Jana (advisor) ; Kozlík, Petr (referee)
Fungus Gloeophyllum trabeum (Agaricomytes: Gloeophyllates) is a brown rot wood-decay fungus which produces a vast spectrum of volatile secondary metabolites. Scientific publications state, that one of the metabolites produced by G. trabeum, can be the substance (3Z,6Z,8E)-dodecatrien-1-ol. This chemical substance is also the main component of trail-following pheromone of Rhinotermitidae termite family. In this diploma thesis, I was trying to verify whether various species of G. trabeum are in fact capable of producing the substance (3Z,6Z,8E)-dodecatrien-1-ol. I was also focusing on the effects of saccharides, present in nutrient solutions, on quantitative and qualitative change in composition of volatile secondary metabolites produced by G. trabeum. The saccharides I used for my research were - maltose, fructose, sucrose, xylose, and mannose. The analysis was made by using comprehensive two-dimensional gas chromatography separation technique with time-of-flight mass spectrometric detection (GC×GC-TOFMS). During my research I discovered that one of obtained species of G. trabeum can produce substance (3Z,6Z,8E)-dodecatrien-1-ol, but only under specific conditions. It is produced when cultivating on Petri dishes on agar - cellulose growth media. The measurement was further validated by...
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